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I hope everyone enjoyed their Spring Break, mine was good but I was working almost the whole week and I came to the lab couple times. This week with the beginning of the spring I finished the graphs for 50% solutions and I compared them to their 100% solution. And Matt went through the graphs and analyzed them for me. He also analyzed the variance and compared all the petri dishes with controlling plates which were the dishes that DI water was their treatment. According to my observation of seeds germination and Matt explanation about the graphs and calculations, I can say that this time I got better result and the result was pretty interesting. I’m sure by looking at the graphs you can find out the differences easily between the graphs. And below are the graphs for all the treatments also the 50% solution treatment for Sycamore, Sun flower and Orange tree.

Radish germination, 100% & 50% solution

Chard germination, 100% & 50% solution

Lettuce germination, 100% & 50% solution

Carrot germination, 100% & 50% solution

Since I made the 50% solution only for Sycamore, Sun flower and Orange tree, Matt decided to do the 50% solution treatment for Oak and Mesquite tree as well to have a complete graphs. And after I get done with that maybe I start the 25% solution for all of them.

So exactly like before the first step was setting up the petri dishes, for this time I just needed to prepare 60 petri dishes; 20 dishes for Oak, 20 for Mesquite and the other 20 are the controlling dishes with DI water. And again I cut off the filter papers, two for each petri dish. Next step was labeling the petri dishes, 5 petri dishes for each seeds (radish, chard, lettuce, and carrot). Then I started to count 25 of each type of seeds and put them on the labeled dishes between the two filter papers. Then for making the solutions I had some grind leaves save since last time and I didn’t need to collect and grind the leaves again, so I weight 10.0 gr of each sample and add 200 mL of DI water to it, I closed the lead with parafilm and let them to stay in room temperature for 24 hours. And finally the day after making the solution, I did water the petri dishes with 5 mL pipet, and covered all of them by parafilm to prevent the evaporation. So I was able to finish all the plates this week and all I need to do is waiting for a week, then start counting the seeds and collecting the data.